Composite

Part:BBa_K5023012:Design

Designed by: Lucas Daniel Ovelar Vargas   Group: iGEM23_UNILA-LatAm   (2023-10-08)


PAR_Ble_SP7_FAST-PETase_Linker_MHETase_SP20_Ter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 704
    Illegal PstI site found at 2129
    Illegal PstI site found at 2920
    Illegal PstI site found at 3298
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 249
    Illegal NheI site found at 1223
    Illegal PstI site found at 704
    Illegal PstI site found at 2129
    Illegal PstI site found at 2920
    Illegal PstI site found at 3298
    Illegal NotI site found at 3309
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1634
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 704
    Illegal PstI site found at 2129
    Illegal PstI site found at 2920
    Illegal PstI site found at 3298
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 704
    Illegal PstI site found at 2129
    Illegal PstI site found at 2920
    Illegal PstI site found at 3298
    Illegal NgoMIV site found at 1085
    Illegal NgoMIV site found at 1146
    Illegal NgoMIV site found at 1438
    Illegal NgoMIV site found at 3236
    Illegal NgoMIV site found at 3368
    Illegal NgoMIV site found at 3398
    Illegal NgoMIV site found at 3965
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed to express and secrete Fast-PETase linked to MHETase in Chlamydomonas reinhardtii, utilizing pJP32 backbone.


Source

Circuit number 2

References